Glucose stimulates the biosynthesis of a human pancreatic islet cell protein detected by an antiserum against the human erythrocyte glucose transporter

[摘要]

An antiserum raised against the purified human erythrocyte glucose transporter specifically immunoprecipitated a major protein of M _r36 000 and a minor protein of M _r 58 000 from human pancreatic islets. The biosynthetic incorporation of [³⁵S]methionine into the 36-kDa protein increased 2–3 fold in 16 mM D-glucose with 0.1 mM IBMX as compared to islets incubated in 3 mM D-glucose either with or without IBMX. D-Glucose tested without or with IBMX had no effect on the biosynthesis of the 58-kDa protein. The 36-kDa protein was also detected in rat islets and rat islet tumor cells. It is suggested that this protein may represent a D-glucose and cAMP sensitive component of a glucose transport or glucose binding protein in the pancreatic β-cell.

[学科分类] 生物化学/生物物理

[关键词] Human erythrocyte glucose transporter;Human pancreatic islet;Glucose transport;β-Cell protein;Glucose regulation;3-Isobutyl-1-methylxanthine;NCS;newborn calf serum;HS;human serum;IBMX;3-isobutyl-1-methylxanthine;FCS;foetal calf serum;HEPES;N-hydroxy-ethyl-piperazine-N-2-ethane-sulfonic acid;PMSF;phenylmethylsulfonyl-fluoride;PCMBS;p-chloromercuribenzenesulfonic acid;SDS;sodium dodecylsulfate;cAMP;cyclic adenosine 3′;5′-monophosphate