Organization of ribosomal DNA in yellow lupine (Lupinus luteus) and sequence of the 5.8 S RNA gene

[摘要]

The large ribosomal repeat of the Lupinus luteus genome is 8.8 kb in length as revealed by Southern blotting and hybridization to 5.8 S rRNA and cloned rDNA fragments of L. luteus and Schizosaccharomyces pombe. It codes for 18 S,5.8 S and 28 S rRNAs. A cloned 3.7 kb EcoR1 subfragment of the rDNA repeat, coding for 5.8 S rRNA and a large portion of the 28 S rRNA, has been sequenced in the region of 5.8 rRNA gene. The predicted rRNA sequence is homologous to broad bean 5.8 S rRNA and can be arranged in the generalized model of 5.8 S RNA secondary structure.

[学科分类] 生物化学/生物物理

[关键词] Lupinus luteus RNA gene;5.8 S RNA;Ribosomal repeat;Ribosomal DNA;Plant gene;DNA sequence;kb;kilobases;rRNA;ribosomal RNA;1 × SSC buffer;0.15 M NaCl;0.015 M sodium citrate (pH 7.0)