Synthesis and biological activity of a fluorescent analogue of 2—5A

[摘要]

A fluorescent analogue of 2—5A, ppp5′ ϵA2′p5′ϵA2′p5′ϵA, was obtained by reaction of 2′, 5′ (pA)₃ with chloroacetaldehyde followed by conversion to the 5-triphosphate through reaction of the corresponding phosphoroimidazolide with pyrophosphate anion. The 5′-monophosphate, p5′ϵA2′p5′ϵA2′p5′ϵA, was not an antagonist of 2—5A action in extracts of mouse L cells. Neither did the 5′-triphosphate, ppp5′ϵA2′p5′ϵA2′p5′ϵA, inhibit translation. Moreover, the 5′-triphosphate was bound to the 2—5A-dependent endonuclease 5000-times less effectively than 2—5A itself.

[学科分类] 生物化学/生物物理

[关键词] Interferon;2—5A;2—5A-Activated endonuclease;Ethenoadenosine;Fluorescence;Oligonucleotide synthesis;ϵA;1;N 6-ethenoadenosine i.e.;(3-β-D-ribofuranosylimidazo[2.1-i]purine);2';5'(pϵA)3;p5′ϵA2′p5′ϵA2′p5′ϵA;2′;5′pp(pϵA)3;ppp5′ϵA2′p5′ϵA2′p5′ϵA;2—5A;ppp5′A2′p5′(A2′p5′) n A;where n⩾1-≈10