The strong hydrophobic domain of the activated estrogen receptor of porcine uterus

[摘要]

Basic estrogen receptor (ER) molecule (vero-ER) of porcine uterus, which was previously shown to be the activated ER necessary to translocate from the cytoplasm into the nucleus, possesses a strongly hydrophobic nature. The strong hydrophobicity of vero-ER was concealed through binding with ER-binding factors (ERBFs). Vero-ER lost its strong hydrophobicity and its capability to bind with ERBFs after limited proteolysis by endogenous protease. The strong hydrophobic domain of vero-ER, indispensable for the nuclear translocation, was assumed to be located near the binding site with ERBFs.

[学科分类] 生物化学/生物物理

[关键词] Estrogen receptor;Hydrophobicity;Activated receptor;Receptor-binding factor;Steroid hormone;Hormone action;ER;estrogen receptor;ERBF;estrogen receptor-binding factor;‘5S’ ER-FF;‘5S’ estrogen receptor-forming factor;TEMA buffer;10 mM Tris-HCl;1.5 mM EDTA;1.5 mM 2-mercaptoethanol;0.25 mM antipain;pH 8.0 at 2°C