This Month's Highlights

[摘要] Some years ago, Weinman’s group purified a novel protein they called sodium-hydrogen exchanger regulatory factor (NHERF). NHERF is now known to be part of a gene family (NHERF-1 and NHERF-2) of docking proteins that assemble multi-protein signaling complexes, including ezrin, the Na/H exchanger present at the proximal tubule apical membrane (NHE3), and protein kinase A through their multiple PDZ domains. These protein-signaling complexes facilitate the phosphorylation of NHE3 by activated PKA, thereby inhibiting its activity. This is a general phenomenon insofar as biochemical association of CFTR with the NHERF PDZ1 domain has also been reported by several groups, and the importance of this interaction for apical sorting of CFTR in polarized epithelia has been demonstrated. In this issue, Weinman’s group extends the reach of NHERF-1 by showing that it is also needed for PTH-mediated phosphorylation of Na/K ATPase, but the mechanism for retrieval of the phosphate transporter NaPi IIa from the apical membrane in response to cAMP does not require NHERF. Page 1711

[学科分类] 泌尿医学

[关键词] Bone marrow necrosis;Sickle cell disease;Hyperhemolysis syndrome