Overestimation of camber and prestress losses for high performance precast concrete bridge girders may discourage the efficient use of longer spans. In this study, construction was monitored on five AASHTO BT-54 girders for Alabama’s HPC Showcase Bridge. Camber and strains from time of prestress transfer to bridge completion were recorded. Thirty-one girders were measured for camber at an overage concrete age of 200 days. Sample cylinders for creep, shrinkage, and modulus of elasticity tests were cast and match-cured during girder production. An incremental time-step analysis was used to predict girder strains, camber, and prestress losses up to the time of deck construction. Field measurements showed good agreement with values calculated with measured materials properties. On average, measured time-dependent midspan losses were 14 percent greater than losses predicted using measured HPC properties. Current analytical techniques can result in accurate predictions of camber and prestress losses for HPC girders if the material properties used in the analysis are representative of the actual concrete used in girder production.