With the increasing use of prestressed concrete for facilities such as offshore structures, it is necessary to know the serviceability behavior in addition to the ultimate behavior of beams subjected to fatigue loading. In this paper an attempt is made to evaluate the influence of fatigue loading on the behavior of partially prestressed concrete beams in flexure. The effects of cyclic creep of concrete and bond deterioration under cyclic loads are considered. The proposed analytical model predicts, from the fatigue properties of the constituent materials, the increase in deflections and crack widths due to repeated loads. The predicted values of deflection and crack width are compared with experimental results. A flow chart and a design example are presented to illustrate the application of the proposed method.