Assay of Glucose-6-phosphate Dehydrogenase in E. coli Cells Ruptured by Phage Ghost

[摘要] The relative activity of glucose-6-phosphate dehydrogenase in E. coli was measured at 340 m¥ì with a spectrophotometer. The synchronized E. coli cells in exponential phase were treated with Phage(T2) ghost, and used as a enzyme solution directly. This assay method supposed to be useful for the continuous determination of enzyme activity in E. coli.

[学科分类] 化学（综合）