Some RNases Involved in the Processing of Bacteriophage T4 tRNA

[摘要] Bacteriophage T4 tRNA processing in E. coli mutant strains defective in RNase ¥², RNase E, and RNase P, respectively, singly or in combinations, was investigated. In RNase E- strains, a RNA band, which would be referred as 9S RNA, accumulates, while in RNase P- strains, lower band of 6S double band is accumulated. In RNase ¥²- strains, the production of tRANGln coded by T4 tRNA gene cluster, is severely depressed and also production of species 1 RNA, which is coded by T4 DNA but not by the tRNA gene cluster, is in somewhat depressed amounts; on the other hand, at the same time, an upper band of 6S double bands, coded by T4 tRNA gene cluster, is accumulated in rather greater amounts as compared to the RNase ¥²+ strain. The upper band RNA of the 6S double band, however, does not appear to be a precursor to the tRNAGln. The present work points to the lack of evidence for an essential cleavage role of RNase ¥², although there must be a role for the RNase ¥² in the T4 tRNA processing.

[学科分类] 化学（综合）