Histidylleucine Hydrolyzing Enzyme : A Dipeptidase From Hog Lung

[摘要] An enzyme capable of hydrolyzing histidylleucine was purified 50 fold from hog lung. The final preparation hydrolyzed 1.6 ¥ìmoles of histidylleucime per minute per mg of protein. The Km of the enzyme for the enzyme was found to be 2¡¿10-4M. The enzyme was required a number of free dipeptides for the substrate specificity, and was inhibited by EDTA and 1,10-phenan-throline. The molecular weight of the enzyme was estimated to be 80,000 daltons from sucrose density gradient sedimentation analysis. The corrected s20,w value was 5.3 S.

[学科分类] 化学（综合）