Microspectrophotometry of Nuclear DNA in Rice Cells Cultured under the Aseptic Conditions

[摘要] Relative amounts of DNA per. nucleus in rice cells, which were produced by aseptic culture, were measured microspectrophotometrically and compared with that in cells of other species of plants. The nuclei were stained by Feulgen reaction using cold hydrolysis technique. The density was estimated by scanning method at 0.5 or l.0 πm line interval with the wave length of 560nm, employing UMSP-1. The rice nuclei had an average diameter of 4.7 pm. They were very small size compared with those of other crops, being about one half of those in oat and corn. The nuclear DNA in rice cells isolated in liquid medium was about 80 per cent of those in root tip cells. While, mung bean had 2.1 times and oat and corn had 12 times in the contents for the rice nuclei. In rice, relative proportions of 4C nuclei was very small in the free cells, but 5 and 13 per cent were observed in the root tip cells and the callus cells, respectively. However 17 per cent in mung bean and 39 per cent in corn were observed for the 4C nuclei.

[学科分类] 农业科学（综合）