Preliminary Investigations on Diagnosis of Strain-Relationship in Corn Plant by Precipitine Test : (3) Relation between fractionated protein and precipitin reactions

[摘要] The present investigation was carried out to ascertain the effect of protein constituents extracted from corn pollen on precipitin reaction. The results obtained are as follows : (1) Fraction analysis of protein contained in the extracts of pollen of the corn plant, var. "Reid's Early Yellow" was carried out. The filtrates were separated into four portions, A, B, C and D; and A contained all fractions of protein and the others contained albumin+globulin-α+globulin-β, albumin+Globulin-α, and albumin, respectively. (2) Rabbits were treated with the filtrates and the antiserum thereby produced were tested by the "Ring" Precipitation with each antigen. (3) The fractions albumin, globulin-β and globulin-γ reacted strongly, while globulin-α reacted weakly.

[学科分类] 农业科学（综合）