STUDIES ON THE DEVELOPMENT OF FRUIT IN SOYBEAN. : (2) Microchemical Observation

[摘要] The materials and their culture method used in this experiment were the same indicated in the previous paper. The samples obtained every day or 2 days interval in the course of fruit-setting were invesigated with the free hand section. The methods of microchemical test used were FEHLING'S and FLUKIGER'S test for reducing sugar, J-J. K. tes for starch, MILLON'S and BITRET test for protein and sudan III test for oil. The results obtained are summarized as follows: (1) Reducing sugare appeared in all tissues of fruit except collenchyma, pulp tissue, scherenchymatous fiber tissue and inter-epidermis of pod in the following order of pod, seed-coat, embryo (cotyledon. radicule and plumule). And it increased gradually in the course of development of embryo. In the ripening stage reducing sugar disappeared in all tissues of pod and seed-coat except the residual of endosperm of seed-coat. (2) Starch was detected in all tissue of fruit except xylem of vascular bundle and lateral vein of pod during 42 days after flowering in the following order of pod, seed-coat, embryo. In the ripening stage it desappeared completely in all part of the fruit except palisade tissue of cotyledon and pith of hypocotyle. (3) Oil appeared in all tissue of pod and seed-coat since 5∼10 days after flowering and in the all part of embryo about 10 days later. On the 48th day after flowering it disappeared in all parts of pod and seed-coat, but it increased gradually in embryo in the progress of ripening. (4) A considerable amount of protein was found in all tissue of fruit in the following order of pod, seed-coat, embryo. It increased by and by all parts of embryo. In the ripening stage it disappeared in all tissues of pod and seed-coat except the residual of endosperm.

[学科分类] 农业科学（综合）