A Microautoradiographic Technique for the Anatomical Studies of Plant Tissue

[摘要] The stripping emulsiom method was tried in order to make the microautoradiograph of the tissue of higher plants. Seminal roots of young corn plants, treated with the solution of H3P32O4 10 microcurries/cc (carrier free) for 18 hours, were made into vertical sections and covered with 1 % celloidin. In a dark room, the stripping emulsion method was tried by using Fuji autoradiographic plate ET-2E, and exposed to β-ray during 16-25 days in a light tight box. At the end of the exposure period, the slides were subjected to photographic development and fixation. There were some possibilities of the loss of radioisotope and also removing during the wet procedure. These losses generally occured to a great extent by the reaction of alcohol, ether and acids. For the prevention of these disadvantages, the fixation and dehydration should be omitted and histological sections should be made by a hand sliding microtome or a freezing microtome. For the same reasons, the staining of the sections should also be omitted. The microscopic photographs obtained from the completed slides, were shown in Fig. 1, 2, 3 and 4.

[学科分类] 农业科学（综合）