Histochemical Investigation on the Accumulation of Phosphorus and Lipid in Aleurone Cell of Rice Kernels

[摘要] The histochemical investigations on localization and properties of inorganic phosphorus, organic phosphorus and lipid were carried out mainly in aleurone layer of rice kernels. The results obtained are fummarized as follows. 1. Three to four days after flowering, inorganic phosphorus was found distributed markedly in dorsal conductive tissues, nucellar epidermis and inner integument. 2. From 5th to 11th days, the differentiation of the aleurone cells became distinct and at the same time the reaction of inorganic phosphorus and organic phosphorus were conspicuous in these cells. On the other hand the reactions became less distinct in epidermis of nucellus and inner integument. From 15th day, phytin phosphate was markedly accumulated in rice brain. At the time of the differentiation of the aleurone grains, the amounts of inorganic phosphorus and organic phosphorus in these grains became the most abundart in all tissues of rice kernel. 3. Organic and inorganic phosphorus were found to be localized in almost the same conductive tissues, but they were present only in small amounts in starch cells. 4. From these results, it is assumed that the inorganic phosphorus and sugar phosphorus, transported into rice kernels from leaves and stems, are translocated via the same conductive tissues. At the first stage, both imported phosphorus stay in the conductive tisses. At the second stage, with the differentiation of aleurone cell and, at the last stage, with the differentiation of aleurone grains, the imported inorganic phosphorus was accumulated in these organs respectively. The sugar phosphorus, at the 2nd and 3rd stages, seems to be transported to starch cells, after the permeation through aleurone cells. Then inorganic phosphorus, liberated in the starch syhthesis, seems to be accumulated in aleurone cells or aleurone grains, via phosphorus body. Phytic acid shosphorus seemes to be synthesized from the inorganic phosphorus in these organs. 5. Three days after flowering, lipids appeared as droplets and were developed in aleurone cell. Lipips were distributed much in outer space of aleurone grains but also found to be contained in these grains.

[学科分类] 农业科学（综合）