Method of Investigation in Rhizobacterial Flora of Crops and Application Case by Using Biolog's Identification System

[摘要] References(15)Cited-By(1)Fifty strains of rhizobacteria were isolated from roots of crops in rotation and monocropping plots of soybeans and sweet potatos one month before harvest, and they were identified by using Biolog's identification system during the period from 1994 to 1997. The strains of 38.3% of the rhizobacteria isolates were identified, and the others were on the process of classification or unidentified. It was assumed that the relative high percentage in unidentification was attributed to insufficient database related to environmental bacteria. If this database ware thoroughly improved, however, this system would be useful for dynamic analyses of rhizobacterial flora in terms of genus or species level, since a large amount of strains of cropes could be smoothly identified with this system. The effects of rotation and monocropping systems on the rhizobacterial flora of crops were evaluated by a diversity index using this system. The diversification of the rhizobacterial flora was not improved in the monocropping plot of soybeans compared with rotation plots of soybeans and sweet potatos and the monocropping plot of sweet potatos.

[学科分类] 农业科学（综合）

[关键词] Biolog's identification system;????;Diversity index;???????;Rhizobacteria;?????;Rhizobacterial flora;??????????;??????