Studies on the Germination of Seeds of Bupleurum falcatum L. : VII. Germination inhibitors of B. falcatum seeds(3)

[摘要] In the previous papers, the germination inhibitors which denoted as inhibitors A and B were isolated from the seed coats of B.falcatum seed. Inhibitor B was obviously superior to inhibitor A in the activities to absorb oxygen and inhibit germination. In the present paper, changes of inhibitors A and B content in seeds were investigated with regard to changes of germination capacity of postharvest. 1. The germination temperature of 15°C was found to bring about high germination capacity and short germination period (days). At 15°C, the germination capacity was low at shedding and grew higher as the time of postharvest advanced up to after shedding. The highest germination capacity was attained at the seeds stored for 5months. Same tendency was observed at 20°C and 25°C. 2. Inhibitors content obviously changed with the period of postharvest. Contents ofinhibitor B were high during early period after seed harvest and lowered as the time of postharvest advanced. On the contrary, contents of inhibitor A lowered were increased as the time of postharvest advanced. 3. Inhibition of germination by exudate from B. falcatum seeds during stratification was observed. Inhibitor B which isolated from the sand used stratification treatment was markedly detected by thin-layer chromatography, although inhibitor A was not clearly. 4. The filter paper used as germination bed on which B. falcatum seeds were placed delayed the germination of lettuce seeds, if B. falcatum seeds were within 5 months after shedding. However, exudate of B. falcatum seeds stored for more than 5 months after shedding was not effected. 5. From the above results, it is possible that changes of dormant depth in B.falcatum seeds may be related to changes of inhibitor B content.

[学科分类] 农业科学（综合）