Isolation and Culture of Protoplast from Finger Millet (Eleusine coracana) Callus

[摘要] High yield of protoplast was obtained from the green callus tissue of finger millet at one week after subculture on MS medium with 0.2 mgl-1 2, 4-D and 0.1 mgl-1 kinetin. The protoplasts were released using the enzyme solution containing 2% Cellulase YC and 0.2% Pectolyase Y-23. About 75% of the protoplasts cultured in the MS liquid medium with 0.4 mgl-1 2, 4-D and 0.1 mgl-1 kinetin supplemented with 5% coconut water were started to show regeneration of the cell wall within 12 h. Cell division was observed within 24 h after culture. The protoplasts that underwent division were about 15%. The sustained cell division could lead to microcolony formation in 4 d after culture. However, further growth did not occur and viability gradually decreased during culture.

[学科分类] 农业科学（综合）

[关键词] Cell division;細胞分裂;Cell wall;細胞壁;Finger millet;シコクビエ;Protoplast isolation and culture;プロトプラストの分離と培養