Histology of Aseptic Callus Tissues Derived from Rice Embryos

[摘要] Histological aspects of growing callus tissue derived from rice embryo have been reported in this paper. Studies with callus tissue were performed with three varieties differing appearence of callus, Cody showing loosely friable callus, Kinmaze showing compact callus, and Te-Tep showing the most compact and huge tissue mass. The growth of rice callus is accompanied by division of layers or groups of meristematic cells in surface region. The callus tissue derived from the surface meristem forms newly friable callus apart from the nurse callus tissue. In the tissue mass showing compact callus, the growth is preceded by activity of both surface and inner meristem accompanied with differentiation and breaking in parenchymatous cells, such as formation of meristematic nodules, tracheid-like cells and lacuna. When grown in a medium containing 10 μM of 2, 4-D any root growth was not observed morphologically but root tip was observed in the inner callus microscopically (fig. 11, 12, 24 and 32). According to these results some discussions were dealt with on histological aspects.

[学科分类] 农业科学（综合）