Transfer and Expression of Small Interfering RNAs in Mammalian Cells Using Lentiviral Vectors

[摘要] siRNA �? small interfering RNA; miRNA �? microRNA; RISC �? RNA-induced silencing complex; shRNA �? small hairpin RNA; dsRNA �? double-stranded RNA; HIV-1 �? human immunodeficiency virus type I; VSV-G �? G protein of vesicular stomatitis virus; CMV �? cytomegalovirus; H1, U6 �? DNA polymerase III promoters

[学科分类] 生物技术

[关键词] lentiviral vectors;shRNA;RNA interference