FURTHER INVESTIGATION ON THE DNA BASE COMPOSITION OF THE GENUS HANSENULA

[摘要] DNA base composition (GC content) of 45 strains of Hansenula and related yeasts reported previously was re-examined by using an improved apparatus for determination of thermal denaturation temperature (Tm) of DNA. In addition to the strains mentioned above, GC contents of 17 strains representing 10 taxa were also investigated.The GC content of species of Hansenula ranged from 32 to 50%, and intrageneric variation was about 18%. The revised polygenetic scheme of Hansenula by Wickerham and the grouping of Hansenula based on proton magnetic resonance (PMR) spectra of mannans were evaluated from the viewpoint of the GC content. Several "natural" groups are deduced in the genus Hansenula on the basis of GC content, antigenic structure, PMR spectra of mannans, and criteria employed in the present system of yeast classification.

[学科分类] 微生物学和免疫学