POLYOL DEHYDROGENASES IN CANDIDA ALBICANS

[摘要] Candida albicans grown in a liquid medium containing 0.5% D-xylose produced an adaptative NADP-polyol dehydrogenase which catalyzes the reversible reduction of some aldoses to the corresponding polyols. This enzyme was extracted from the culture and purified 134-fold using ammonium sulfate precipitations followed by calcium phosphate gel column chromatography. The Michaelis constant for D-xylose was determined to be 6.7×10- 3M. Heating of the enzyme up to 43° enhanced its activity, but it became completely inactive at 65°.

[学科分类] 微生物学和免疫学