Rhizobium kunmingense sp. nov., isolated from rhizosphere soil of Camptotheca acuminata Decne

[摘要] Strain LXD30T was isolated from rhizosphere soil of a plant of the species Camptotheca acuminata Decne which is native to warm, humid stream banks in southern China. Analysis of the 16S rRNA gene sequence revealed that the Gram-negative, rod-shaped bacterium fell within the realm of the genus Rhizobium and was most closely related to Rhizobium huautlense SO2T (96.4% sequence similarity) and Rhizobium cellulosilyticum LMG 23642T (96.4%). The isolate grew optimally at pH7.0 and 25-28ºC in the presence of 0-1% (w/v) NaCl. Major fatty acids were C16:0 (17.5%) and summed feature 7 (C18:1ω7c/ω9t/ω12t, 58.3%). Unequivocally low 16S rRNA (<97%), recA (<92%) and atpD (<90%) gene sequence similarities to all existing species of the genus and phenotypic characteristics all suggested that strain LXD30T (=KCTC 22609T=CGMCC 1.8903T) represents a novel Rhizobium species, for which the name Rhizobium kunmingense sp. nov. is proposed.

[学科分类] 微生物学和免疫学

[关键词] atpD gene;polyphasic taxonomic;recA gene;Rhizobium kunmingense sp. nov.;16S rRNA gene