OXIDATIVE DECARBOXYLATION OF 6-PHOSPHOGLUCONATE IN HETEROLACTIC BACTERIA

[摘要] 1. Enzymatic studies were made on the oxidative decarboxylation of 6-phosphogluconate in typical heterolactic acid bacteria, and it was concluded that mechanism of the oxidative decarboxylation of 6-phosphogluconate in these bacteria can be classified into three types as follows:(1)6-phosphogluconate+NAD→ribulose 5-phosphate+CO2+NADH2(2) 6-phosphogluconate+NAD→2-keto-6-phosphogluconate+NADH2 2-keto-6-phosphogluconate→ribulose 5-phosphate+CO2(3) mixed reactions of (1) and (2).2. It was found that 6-phosphogluconate dehydrogenase (decarboxylating) [EC 1.1.1.44] obtained from Lactobacillus brevis ATCC 8281 consists of 6-phosphogluconate dehydrogenase (not decarboxylating) [EC 1.1.1.43] and 2-keto-6-phosphogluconate decarboxylase.

[学科分类] 微生物学和免疫学