COMPARATIVE STUDIES OF DNA POLYMERASE I OF ENTERIC BACTERIA

[摘要] DNA polymerases I of several enteric bacteria were partially purified and their biochemical, antigenic properties and ability to replicate the DNA of colicine El plasmid (Col El) with the extracts of Escherichia coil pol A were examined. The results suggested that all polymerases had almost the same molecular weight and pH optimum and showed resistance to araCTP and NEM. They also possessed both 3′-5′- and 5′-3′-exonucleases. The antigenicities and the ability to replicate Col El DNA, however, varied with each polymerise. Each polymerase seems to be composed of strongly conserved structure and of the structure which varies from genus to genus. The species-specificities observed in the polymerases seem to reflect the phylogenic relations of bacteria.

[学科分类] 微生物学和免疫学