NITRATE REDUCTION IN BACTEROIDES MULTIACIDUS: ITS EFFECT ON GLUCOSE METABOLISM AND SOME PROPERTIES OF THE REDUCTASE

[摘要] Bacteroides multiacidus fermented glucose to lactate and succinate as major products and produced small amounts of acetate and pyruvate. During nitrate reduction cell growth increased slightly and the formation of succinate and lactate decreased while acetate increased.The level of nitrate reductase activity in the extracts was several times higher in cells grown on nitrate, while the fumarate reductase level was lowered compared with cells not grown on nitrate. In extracts, nitrate reductase and fumarate reductase occurred mainly in a particulate fraction, whereas nitrite reductase was mostly in a soluble fraction. Addition of tungstate to the culture medium decreased the nitrate reductase activity as well as nitrite formation, suggesting that the enzyme is a molybdoprotein. Ferredoxin from Clostridia and viologen dyes served as electron donors for nitrate reduction. A c-type cytochrome with peaks at 557.5, 527, and 423nm in the extract was partly reduced by NADH and oxidized by fumarate, and to a lesser extent also by nitrate, even in extracts from cells grown in the presence of nitrate. Km′s for substrates and pH optima were also reported for the reductases in crude extracts.

[学科分类] 微生物学和免疫学