PURIFICATION AND PROPERTIES OF CYTOCHROME c FROM METHYLOMONAS METHANICA

[摘要] Soluble cytochrome c from Methylomonas methanica was purified to homogeneity and its properties were characterized. The molecular weight as determined by gel filtration is 18, 000 and the subunit size as determined by sodium dodecyl sulfate gel electrophoresis is 17, 000. The amino acid composition and spectral properties of cytochrome c are presented. Antisera prepared against the purified cytochrome from Methylomonas methanica cross-reacted with cytochrome c in cell extracts of various obligate methane-utilizing bacteria.

[学科分类] 微生物学和免疫学