ALGINATE PRODUCTION BY AZOTOBACTER VINELANDII IN BATCH CULTURE

[摘要] In laboratory study, some factors influencing the secretion of alginic acid by highly mucoid strain of Azotobacter vinelandii were investigated in batch culture. The highest alginate yields were (5.5-6.2mg ml-1 culture supernatant) obtained for growth in a nitrogen-rich and phosphate-limited medium with sucrose as carbon source, aerated by shaking at 240 rpm. At 140rpm, alginate yield was only 1.6mg ml-1. If sucrose was replaced by glucose, both growth and alginate production ware reduced.

[学科分类] 微生物学和免疫学