SPECTROPHOTOMETRIC CHARACTERIZATION OF THE GENUS ACTINOMYCES

[摘要] Twenty-two strains of Actinomyces bovis, Actinomyces israelii, Actinomyces naeslundii, Actinomyces odontolyticus, Actinomyces viscosus, and Arachnia propionica were studied by whole cell infrared spectrophotometry. Different spectra were obtained with the cultures grown in brain heart infusion medium and those grown in glycerol Kelner-Morton medium under anaerobic condition. The spectra measured with the test organisms cultivated in glycerol Kelner-Morton medium were compared with those of Streptomyces (Actinomadura), Nocardia, andMycobacterium spp. described previously. All strains of microaerophilic actinomyces showed absorption pattern of Streptomyces (Type B) or Nocardia (Type C) in region I, and that of Streptomyces (Type A) in region II, and absorption pattern of Mycobacterium (Type E) in region IV. An absorption pattern specific to this group of organisms was observed in region III, 1460 to 1385cm-1.

[学科分类] 微生物学和免疫学