TRANSFORMATION OF CORTISOL WITH REPRESENTATIVE FUNGI

[摘要] The ability of different locally isolated fungi to transform cortisol to its Δ1-dehydro derivative (prednisolone) was investigated. The desired reaction was performed by some organisms; namely, Fusarium oxysporum 153, F. moniliforme 2 and 157, F. solani, Gliocladium sp., and Helminthosporium speciferum. Reduction of the 20-keto group of prednisolone was observed with F. oxysporum 153, F. moniliforme 2, and Gliocladium sp.Study of the convertibility of cortisol with the most active fungus (F. oxysporum 153) revealed the existence of Δ1-dehydrogenase and 20β-keto reductase systems which exhibit different velocities and activities. The dehydrogenation reaction proceeds faster during the early stages of fermentation, while the reductase system attains its maximal activity at later stages of the transformation period.

[学科分类] 微生物学和免疫学