The FPase properties and morphology changes of a cellulolyticbacterium, Sporocytophaga sp. JL-01, on decomposing filter papercellulose

[摘要] Sporocytophaga sp. JL-01 is a sliding cellulose degrading bacterium that can decompose filter paper (FP), carboxymethyl cellulose (CMC) and cellulose CF11. In this paper, the morphological characteristics of S. sp. JL-01 growing in FP liquid medium was studied by Scanning Electron Microscope (SEM), and one of the FPase components of this bacterium was analyzed. The results showed that the cell shapes were variable during the process of filter paper cellulose decomposition and the rod shape might be connected with filter paper decomposing. After incubating for 120 h, the filter paper was decomposed significantly, and it was degraded absolutely within 144 h. An FPase1 was purified from the supernatant and its characteristics were analyzed. The molecular weight of the FPase1 was 55 kDa. The optimum pH was pH 7.2 and optimum temperature was 50°C under experiment conditions. Zn2+ and Co2+ enhanced the enzyme activity, but Fe3+ inhibited it.

[学科分类] 微生物学和免疫学

[关键词] cellulose decomposition;FPase;Scanning Electron Microscope;Sporocytophaga