STUDIES ON LIGHT SCATTERING SPECTRUM OF MICROBIAL CELLS IN SUSPENSION

[摘要] By using monocellular microbes of spherical or quasi-spherical shape, spectrophotometrical studies on turbidity of cell suspension were made with the following results.(1) Commercial spectrometers designed for solution chemistry can be used for approximate measurement of absolute turbidity of microbial suspension.(2) Light scattering spectra of microbial suspensions could thereby be easily obtained making rossible "light scattering analysis" based on the Rayleigh-Gans' and Hart-Montroll's equations.(3) General procedures for obtaining cellular index of refraction, cell volume and cell population were examined in the case of Saccharomyces cereuisiae and Chrorella ellipsoidea.(4) "Light scattering analysis" gave some information on biological phenomena such as cell growth and osmosis in liquid culture.(5) Variations of cellular index of refraction caused by volume change of cells explained the apparent inversion of turbidity change observed in osmosis of microbial suspension.

[学科分类] 微生物学和免疫学