STUDIES ON ATP DEAMINASE

[摘要] The distribution of deaminases active for adenosine compounds was studied and it was found that besides two kinds of deaminases, i.e., non-specific ATP and adenosine deaminases, another acid-type ADP-deaminating enzyme was present in Aspergillus species.Deaminases from Aspergillus glaucus and A. repens were purified and it was confirmed that they were of the same type of non-specific ATP deaminase which was found in Microsporum audouini by the authors.Non-specific acid ADP-deaminating enzymes were found and purified from the koji cultures of both A. ochraceus and A. melleus. They deaminate ADP, ATP, adenosine sulfate, adenosine tetraphoshate, 5′-AMP, dAMP, 3′, 5′-cyclic AMP, adenosine, and 3′-AMP in this order. The optimum pH of ADP-deaminating enzyme obtained from both strains were 3.4 in acetate buffer, and the Km values of A. ochraceus and A. melleus were calculated to be 4.0×10-5M and 6.6×10-5M, respectively.

[学科分类] 微生物学和免疫学