Características das células parasitadas pelo Trypanosoma cruzi na parede da veia central das supra-renais de Chagásicos crônicos

[摘要] Some morphologic aspects of the smooth muscle cells, parasitised by T. cruzi in the adrenal vein of chronic chagasic patients were studied. The staining techniques used were the following: Hematoxylin and Eosin (HE), PAS, Feulgen and the peroxidase antiperoxidase (PAP) immunocyto chemical method for identification of T. cruzi antigen. The intracellular amastigotes were often surrounded by a clear halo and the cytoplasm of the parasitised smooth muscle cells were granular or reticular in appearance being basophil and, PAS and Feulgen negative. These data suggest that the cytoplasmatic basophil material could be ribosomic RNA. The intracellular nests of amastigotes were surrounded by a PAP positive "membrane-like" structures. This PAP positive "membrane-like"material, as well assome cytoplasmatic PAP positive granulomatous material, could be due to absorbed antigensfrom the parasite or to T. cruzi- like antigens resultedfrom the interaction between the parasite and the host cell.

[学科分类] 农业科学（综合）

[关键词] Trypanosoma cruzi;Chagas' disease;Smooth muscle of the central suprarenal vein