Comparative pharmacokinetic and tissue distribution study of baicalin, baicalein, wogonoside, wogonin and oroxylin-A after oral administration of Component compatibility of SHT and total flavonoids fractions of Radix scutellariae to rats

[摘要] Sanwu-Huangqin-Tang (SHT) is a classical prescription used for treatment of gynecological disease, and its key ingredient is Radix scutellariae (Scutellaria baicalensis Georgi, Labiatae). Baicalin, wogonoside, baicalein, wogonin and oroxylin-A are five main effective ingredients enriched in Radix scutellariae. In the present study, pharmacokinetic and tissue distribution difference of the five compounds following oral administration of Component Compatibility of Sanwu-Huangqin-Tang (CCSHT) and total flavonoids fractions of Radix scutellariae (FSR) were investigated in male Sprague-Dawley rats with approximately the same dose. The amount of flavonoids in plasma and tissues were measured by a rapid and sensitive HPLC-MS/MS method. Unpaired Student's-test was used for statistical comparison. The bimodal phenomenon was observed in plasma profile after oral administration of FSR and CCSHT. Statistical significant increase (P < 0.05) in pharmacokinetic parameters (including Cmax and AUC0→t) and tissue distribution of the target compounds were observed after oral administration CCSHT comparing with FSR, but there were no significant differences between the two groups in parameters of Tmax. The results indicated that compared with FSR, the bioavailability and distribution amount of flavonoids could be greatly improved by co-administrating alkaloids in Radix sophorae flavescentis and polysaccharide in Radix Scutellariae.

[学科分类] 分析化学