Matrix solid phase dispersion extraction for determination of flavonoids in the flower of Chrysanthemum morifolium Ramat. by capillary zone electrophoresis

[摘要] Matrix solid phase dispersion was applied for the extraction of flavonoids, including kaempferol, apigenin, naringenin, (+)-catechin hydrate, rutin hydrate, quercetin, luteolin and ferulic acid in the flower of Chrysanthemum morifolium Ramat. The separation and determination of the target analytes were performed by capillary zone electrophoresis. Florisil was used as a dispersant and methanol was used as the elution solvent. The elution solvent volume and flow rate were 8 mL and 0.5 mL min−1, respectively. The limits of detection for determining the analytes were in the range of 46.49–72.03 ng mL−1. The relative standard deviations varied between 1.41% and 3.40% (n = 5). Real samples were analyzed. The recoveries for determining the flavonoids were in the range of 80.13–121.25%. The results show that the present method is feasible for determining flavonoids in the flower of Chrysanthemum morifolium Ramat.

[学科分类] 分析化学