Determination of aristolochic acids in rat serum by high performance liquid chromatography-Q-TOF tandem mass spectrometry

[摘要] Four aristolochic acids (AAs) including AA-I, AA-II, AA-IIIa and AA-IVa in rat serum were simultaneously determined by HPLC-Q-TOF-MS. A new solid phase extraction (SPE) resin with reversed-phase/strong anion exchange mixed groups (C18-SAX) was used to effectively purify and enrich the analytes, which demonstrated much better performance in comparison with common C18-SPE resin in terms of noise level and recovery. The chromatographic separation was performed on a C18 column under isocratic elution, and the effluent was analyzed by TOF-MS-MS in the mode of positive multiple reaction. Naproxen was used as the internal standard (IS). The limit of detection was 3, 6, 0.09 and 1.5 ng mL−1 for AA-I, AA-II, AA-IIIa and AA-IVa, respectively. Intra- and inter-day precisions measured as relative standard deviation of retention time were less than 1.1% and 1.5%, respectively.

[学科分类] 分析化学