Anthracene appended coumarin derivative as a Cr(III) selective turn-on fluorescent probe for living cell imaging: a green approach towards speciation studies

[摘要] A facile one step reaction between 6-aminocoumarin and anthracene-9-carboxaldehyde has resulted in a novel fluorescent probe, 6-((anthracen-9-yl) methyleneamino)-2H-chromen-2-one (AC) which is capable of selectively detecting intracellular Cr3+ in contaminated living cells under a fluorescence microscope. The binding of AC with Cr3+ is well characterized by different spectroscopic techniques. The binding constant values in CH3CN–water (9:1, v/v) and methanol–water (9:1, v/v) solutions have been estimated to be 1.1 × 105 and 3.1 × 105 respectively using the Benesi–Hildebrand equation. The detection limit for Cr3+ is 0.5 × 10−6 M. The developed method has been used for speciation studies in a fast and environment friendly way using least amounts of samples, non-hazardous chemicals and solvents.

[学科分类] 分析化学