Application of frontal affinity chromatography combined on-line with mass spectrometry to screening PAI-1 inhibitors from traditional Chinese medicine

[摘要] Frontal affinity chromatography combined on-line with mass spectrometry (FAC-MS) is an efficient method for screening traditional Chinese medicine (TCM). This paper reports the application of FAC-MS to screening PAI-1 inhibitor from TCM. Novel small molecule inhibitors derived from natural products are particularly desirable as inhibitors of PAI-1. Several promising inhibitors of PAI-1 from TCM were identified by FAC-MS. These inhibitors included chrysophanol, rhein, corilagin, and geraniin, with 50% inhibitory concentrations (IC50) of 1.06, 37.32, 30.38, and 10.48 μM, respectively. The results indicate that FAC-MS is a direct and fast method for screening active compounds from complex molecular systems. The sample in this method is a mixture, so it is suitable for the screening Chinese herb extracts.

[学科分类] 分析化学