Screening of metal-resistant coal mine bacteria for biofabrication of elemental silver nanoparticle
[摘要] Green synthesis approaches for nanoparticle synthesis are considered as nontoxic, eco-friendly and cost-effective approaches than other physical and chemical approaches. Here, we report green synthesis of silver nanoparticle using the bacteria from the habitat of relatively metal-rich coal mine dust. The bacteria showed resistance to significant concentration of anti-microbial Ag(I) ion. The nanoparticle was synthesized at minimum inhibitory concentration (MIC) of Ag(I) using only two isolates. The synthesis of silver nanoparticle was confirmed from the surface plasmon resonance property of the nanoparticle, whereas morphological features were studied using field emission scanning electron microscope. The hydrodynamic size analysis showed the presence ofmicroparticles along with the nanoparticles. Additionally, IR study provided information about the bacterial proteins involved in either reduction of Ag(I) into silver nanoparticle or capping of reduced silver nanocrystal or both.Thus, majority of the bacteria found in the coal mines have the resistance against the antimicrobial metal ion, and the potential to reduce the ion into nano- or micro-particles. Hence, the bacteria can be used as a single cell factoryfor production of silver nanomaterial.
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[效力级别] [学科分类] 材料工程
[关键词] Green synthesis;nanoparticles;bacteria;bacterial interface;MIC. [时效性]