The influence of dual CYP17 expression on adrenal steroidogenesis in the South African Angora Goat

[摘要] This study describes:• the cloning and sequencing of cytochrome P450 17 -hydroxylase/17,20lyase (CYP17), 3 -hydroxysteroid dehydrogenase (3 HSD) andcytochrome b5 from the South African Angora goat;• the identification of two CYP17 genes encoding two unique CYP17isoforms in the Angora goat;• the development of a UPLC-APCI-LC method for the separation andquantification of seven adrenal steroids;• the characterisation of the enzymatic activity of the two Angora CYP17isoforms expressed in non-steroidogenic COS-1 cells. The Km and Vvaluesfor the metabolism of pregnenolone and progesterone weredetermined;• the development of a rapid and accurate real-time PCR genotyping test forCYP17 in Angora goats. Three unique genotypes were identified;• the determination of blood cortisol levels upon the stimulation of the HPAaxisby intravenous insulin injection in the three Angora goat genotypes.

[发布机构] Stellenbosch University