Mycobiota ofLupinus albusseeds from a public germplasm collection

[摘要] Seedborne mycobiota ofLupinus albuswas assessed using blotter paper and agar media with Rose Bengal or semi-selective media forPythiumorFusarium . Samples of 200 seeds were taken from each of 16 inventories, comprising 14 accessions originating from Germany, France, Ukraine, Syria, Hungary or Spain, and increased 1-2 times through propagation by the USDA-ARS Western Regional Plant Introduction Station. A total of 307 fungus isolates were recovered and preserved with over 96% belonging to 10 genera:Penicillium(47%),Cladosporium(32%, includingCladosporium -likeChalastospora ) , Alternaria(4%),Aspergillus(4%),Fusarium(3%),Rhizopus(3%) , Ulocladium(3%),Chaetomium(0.5%) andArthrinium(< 0.5%). By sub-sampling, fungi were further identified by morpho-cultural characters and/or DNA sequences asAlternaria infectoria ,A. alternata,andA. tenuissimaspecies-groups,Arthrinium arundinus ,Aspergillus niger ,Chalastospora gossypii ,Cladosporium herbarumandC. cladosporioidesspecies complexes,Chaetomiumsp.,Fusarium acuminatum ,Rhizopus stolonifer , andUlocladium atrumgroup.PenicilliaincludedP. hordei , but mostPenicilliacould not be assigned species names either by morphological traits or β-tubulin DNA sequence data.

[学科分类] 植物学

[关键词] germplasm collection;lupin;lupine;Lupinus;mycobiota;mycoflora;plant pathogen;seed borne;seed health