Identification of two CYP17 alleles in the South African Angora goat

[摘要] ENGLISH ABSTRACT:This study describes:1. The isolation of total RNA and mRNA from Angora goat adrenals.2. Synthesis and nucleotide sequence alignment of Angora goat CYPI7 cDNA. Two DNAsequences were produced, identifying two CVP 17 alleles in an Angora goat from theSwartland district.3. The development of a CYPI7 genotype test for Angora goats.4. Genotyping of Angora goats and Boer goats with the developed genotype test.S. Mapping of the substituted amino acids in the amino terminal of CVP 17 to a specificCYPI7 genotype.6. Partial synthesis and alignment of Angora goat genomic nucleotide CYPI7 sequences.

[发布机构] Stellenbosch University