Different Prostanoids Are Involved in Bradykinin-Induced Endothelium-Dependent and -Independent Vasoconstriction in Rat Mesenteric Resistance Arteries

[摘要] References(26)Cited-By(2)Mechanisms underlying bradykinin-induced vasoconstriction were investigated in rat perfused mesenteric vascular beds with active tone. In preparations with intact endothelium, bolus injections of bradykinin (1 to 1,000 pmol) dose-dependently produced three-phase vascular effects, which consisted of a first-phase vasodilation followed by a second-phase vasoconstriction and a subsequent third-phase vasodilation; these effects were abolished by FR172357 (bradykinin B2-receptor antagonist), but not by des-Arg9-[Leu8]-bradykinin (bradykinin B1-receptor antagonist). In preparations with intact endothelium, indomethacin (cyclooxygenase inhibitor), seratrodast (thromboxane A2 (TXA2)-receptor antagonist), ONO-3708 (TXA2/prostaglandin H2 (PGH2)-receptor antagonist) or ozagrel (TXA2 synthesis inhibitor) markedly inhibited the bradykinin-induced vasoconstriction. In preparations without endothelium, the bradykinin-induced vasoconstriction was abolished by indomethacin and ONO-3708, while seratrodast and ozagrel had no effect. These results suggest that the endothelium-dependent vasoconstriction of bradykinin is mainly mediated by TXA2 and that prostanoids other than TXA2, probably PGH2, in mesenteric vascular smooth muscle are responsible for bradykinin-induced endothelium-independent vasoconstriction.

[学科分类] 药学

[关键词] bradykinin;vasoconstriction;thromboxane A2;prostaglandin H2;rat mesenteric resistance artery