Forefront of Na+/Ca2+ Exchanger Studies: Stoichiometry of Cardiac Na+/Ca2+ Exchanger; 3:1 or 4:1?

[摘要] References(21)Cited-By(11)The stoichiometry of the Na+/Ca2+ exchanger (NCX) had been generally accepted as 3 Na+:1 Ca2+. However, recently a challenging stoichiometry of 4:1 was proposed. Therefore, using guinea pig ventricular cells, we re-examined the stoichiometry by measuring the reversal potential of the NCX current and intracellular Ca2+ concentrations under the whole-cell voltage clamp. We confirmed that the stoichiometry of NCX is 3:1 not 4:1. In addition, we explored the possible reasons for obtaining erroneous results of a 4:1 stoichiometry.

[学科分类] 药学

[关键词] Na+/Ca2+ exchanger (NCX);stoichiometry;cardiac myocyte