A Low-Cost Method for Brain Slice Cultures

[摘要] References(5)Cited-By(24)Low-cost, simple procedures for organotypic tissue cultures are desirable for high-throughput biological experiments such as large-scale medical/drug screening. We present a practical and economical method to cultivate brain slices using hydrophilic filtration membranes. With a cost reduction of more than 90%, this technique allows us to prepare hippocampal slice cultures that are morphologically and functionally indistinguishable from those obtained by the widely used Millicell-CM® method.

[学科分类] 药学

[关键词] membrane-interface tissue culture;organotypic culture;hippocampal slice