Differences in α1-Adrenoceptor Subtype-Mediated Vasoconstriction by Tyramine and Nerve Stimulation in Canine Splenic Artery

[摘要] References(30)This study was designed to clarify the α1-adrenoceptor subtypes mediating the vasoconstrictor response to tyramine in isolated and perfused canine splenic artery. It was shown that tyramine potentiated the nerve stimulation-induced second peaked vasoconstriction that was readily suppressed by prazosin treatment. A bolus injection of tyramine (0.01 - 0.3 μmol) caused a vasoconstriction in a dose-related manner. The tyramine-induced vasoconstriction was inhibited by WB 4101 (10 and 100 nM), an α1A-and α1D-adrenoceptor antagonist, in a concentration-related manner. Neither BMY 7378 (100 nM), a selective α1D-adrenoceptor antagonist, nor chloroethylclonidine (60 μM), an α1B- and α1D-adrenoceptor antagonist, affected the tyramine-induced response. The results indicate that the noradrenaline released by tyramine may diffuse to the extrajunctional cleft, and thus it activates the extrajunctional α1A-adrenoceptors, because nerve stimulation-evoked second peaked vasoconstrictions were markedly inhibited by chloroethylclonidine but not by WB 4101.

[学科分类] 药学

[关键词] α1A-adrenoceptor;tyramine;splenic artery;canine;vascular neuroeffector transmission