Effects of neutralising interleukin-6 on glucocorticoid-mediated adaptations to stress in rat skeletal muscle and liver

[摘要] ENGLISH ABSTRACT: This study (2 x 2 factor design) describes an investigation into the physiological interactionbetween the peripheral endocrine and cytokine systems after the organism has beenexposed to psychological stress. An in vivo rodent model with two interventions was used:(1) mild psychological stress (immobilisation for 2 hours per day, for 4 days); (2) an antiinterleukin(IL)-6-antibody injection. Thirty-nine male Wi star rats were divided into 4 groupsand given either the antibody (CA, control antibody) or stress (IP, immobilisation placebo),or both (IA, immobilisation antibody), or neither (CP, control placebo). Antibody andplacebo (saline) were injected intraperitoneally. Differences between groups for thefollowing parameters were determined in blood or metabolic tissues, viz. skeletal muscleand liver:1) corticosterone concentrations,2) glucocorticoid receptor (GR) binding capacity and3) activities of metabolic enzymes, tyrosine aminotransferase (TAT) andglutamine synthetase (GS).Groups lP and lA showed a significant loss in body mass (CP vs. lP, p

[发布机构] Stellenbosch University