Objective: in this systematic reviewthe aim was to summarise the in vivo/in vitro evidence on therole of oxidative-nitrosative stress in pathogenesis of dengue.
Methods: we searched electronicdatabases (PubMed, EMBASE, The COCHRANE library, ScienceDirect, Scopus, SciELO,LILACS via Virtual Health Library, Google Scholar) using the term: dengue, denguevirus, severe dengue, oxidative stress, nitrosative stress, antioxidants, oxidants,free radicals, oxidized lipid products, lipid peroxides, nitric oxide, and nitricoxide synthase. Articles were selected for review by title and abstract excludingletter, review, epidemiological studies, and duplicates studies. Selected articleswere reviewed for used animal model or cell cultures, original purposes, strainof virus or type of antibody, main outcomes, methods, and oxidative-nitrosativestress markers values.
Results: in total, 4330 non-duplicatesarticles were identified from computerized searches of reference databases,of which 32 were eligible for full text searching. The results of in vivostudies were obtained from monkey and knockout and/or wild-type mice. Humanperipheral blood mononuclear cells were cell cultures most commonly used inidentified in vitro studies, following by human endothelial cells cultures.DENV-2 strains were most used.
Conclusions: in conclusion, a largebody of in vivo and in vitro evidences showed that oxidative/nitrosativestress can be related to production of pathogenesis-related protein, increasedsusceptibility of mice to DENV infection, hemorrhage development in mice, proinflammatorycytokines and transcriptional factor expression, and DENV replication in variouscell cultures.
