Detection of beta-exotoxins in isolates of Bacillus thuringiensis native to Cuba

[摘要] Introduction: Bacillus thuringiensis is a sporulated gram-positive bacterium, well known mainly for its pathogenic action against insects. Some strains synthesize thermostable nucleoside analog secondary metabolites identified as beta-exotoxins. The World Health Organization has recommended that beta-exotoxin-producing Bacillus thuringiensis strains not be used for insect control, due to their unspecific range of action and their toxicity to mammalian cells.Objective: detect by means of bioassays the presence of beta-exotoxins in Cuban isolates of Bacillus thuringiensis with entomopathogenic activity against Aedes aegypti (Linneaus, 1762).Methods: aedes aegypti eggs were put in 500 mL water at 37 °C until eclosion. Next, 66 mg fish flour were added to 10 mL of the supernatant of the autoclaved culture. Living individuals were counted daily for 7 days. Statistical processing of mortality data was conducted with one-way analysis of variance (ANOVA) and Tu

[学科分类] 传染病学

[关键词] Bacillus thuringiensis;beta-exotoxins;bacterial toxins;bioassays;Aedes aegypti